Re: [Histonet] plastic embedded bone sections

From:louise renton

We routinely cut human and non-human primate bones embedded in 9100
new on an automated Leica SM2500 microtome ( not familiar with the
microm model - is it automated?). We use a tungsten carbide tipped
knife to cut 6mu sections. I
best regards

On 2/9/06, Michael J Voor  wrote:
> Dear Histonet,
> We are trying to section PMMA (Technovit 9100 New) embedded
> undecalcified bone samples (rat tibias).  We plan to use a Microm
> sliding microtome.  We need advice on exactly what blade to use and what
> blade angle would be best to create sections on the order of 7-10
> microns.  Thanks for any help.
> Michael J. Voor, Ph.D.
> Orthopaedic Bioengineering Laboratory
> Director of Research
> Department of Orthopaedic Surgery
> University of Louisville
> Louisville, KY  40292
> (502) 852-7673  FAX: (502)852-7227
> e-mail:
> _______________________________________________
> Histonet mailing list

Louise Renton
Bone Research Unit
University of the Witwatersrand
South Africa
"Does a conceited cowboy, only ride on pompous grass?.

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