We routinely cut human and non-human primate bones embedded in 9100
new on an automated Leica SM2500 microtome ( not familiar with the
microm model - is it automated?). We use a tungsten carbide tipped
knife to cut 6mu sections. I
best regards

On 2/9/06, Michael J Voor  wrote:
> Dear Histonet,
>
> We are trying to section PMMA (Technovit 9100 New) embedded
> undecalcified bone samples (rat tibias).  We plan to use a Microm
> sliding microtome.  We need advice on exactly what blade to use and what
> blade angle would be best to create sections on the order of 7-10
> microns.  Thanks for any help.
>
>
> Michael J. Voor, Ph.D.
> Orthopaedic Bioengineering Laboratory
> Director of Research
> Department of Orthopaedic Surgery
> University of Louisville
> Louisville, KY  40292
> (502) 852-7673  FAX: (502)852-7227
> e-mail: mike.voor@louisville.edu
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"Does a conceited cowboy, only ride on pompous grass?.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet