Re: [Histonet] paraffin embedding of frozen tissue

From:Rene J Buesa

Carmen:
  We used to have a frozen tumors bank (tissues preserved at -80║C) and when some study was going to be done we either cut frozen sections, or processed as regular tissues.
  Either way you have to fix it (as if it were a "fresh" tissue) and processed it with any regular protocol suitable for the type of tissue.
  Now, for immunohistochemistry (IHC), since you have fixed it in formalin, you will have to perform heat induced epitope retrieval before the IHC.
  We used to do the same thing for the tissues left-over from the frozen sectioning diagnostic procedure.
  Hope this will help you.
  RenÚ J.

Carmen Contreras-Sesvold  wrote:
  Greetings histonetters,
I have recently found that sectioning frozen lung is a bit difficult. I was wondering if anyone has ever paraffin embedded previously fixed (and cryproserved) frozen sections? And if so what were the results? I am performing immunohistochemistry / immunofluorecent staining on mice lung tissue and I was just wondering if it was possible. I also encourge advice on the subject matter.


I have learned quite a bit from this web site.
Thank you very much.
Carmen Contreras-Sesvold

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