One possibility is that the sections do not
contain the antigen you are trying to detect. Do
you have simultaneously processed known positive
control sections?

John Kiernan
Anatomy, UWO
London, Canada.
------------------------------
"Horn, Diane A" wrote:
> 
> 
> I am currently doing IHC on develping mouse teeth and I am getting only backgroung staining.  The tissues are fixed O/N in 4% paraformaldehyde @4 degrees and embedded in paraffin.  I have cut my sections @ 4um.  I have tried HIER with Vector unmasking solution (boiling) for 2 minutes and using a kit for PCNA from Zymed.  Has anyone done IHC on teeth that could give me some advice on what to try next?
> 
> Thanks!
> Diane
>

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