Re: [Histonet] B-Gal endogenous expression
Dimri et 10 al (1995) Proc. Natl Acad. Sci. USA
Debacq-Chainlaux et 11 al (2005) J. Cell Sci. 118:
The lysosomal enzyme has a pH optimum about 4.
Senescent cells contain a different enzyme active
about pH 6. (The enzyme of the lac-Z reporter gene
works at pH 7+ so it's not confused with the
lysosomal enzyme activity.) In the major earlier
papers on galactosidase histochemistry
(1960s-'70s) the illustrations mainly showed
intestine, with strong staining in epithelial
cells; also renal tubules if I remember rightly.
Hope this helps.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
"Andrea T. Hooper" wrote:
> Does anyone have references or anecdotal experience as to where
> mammalian B-Gal is endogenously expressed if anywhere in mouse or
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