Re: [Histonet] A multi-use simple blocking solution?....
We prefer to use normal serum blocking, with the serum matched to the host
of the secondary and do not use BSA based blockers. This has been
discussed at great lengths on Histonet, check out Histonet
Archives. Blocking, per your question, is done many ways.
I suggest you do to the DAKO website, information and access the Handbook
of Immunochemical Methods, Boesnish discusses various blocking agents, and
you can download this wonderful freebie in pdf format.
You can buy superblocks ready to use from various companies, i.e.At 03:13
PM 2/9/2006, you wrote:
>I have been looking through the protocols on some of the IHC databases for
>blocking solutions. Some of the recipes are fairly exotic (fish skin
>gelatin?). Is there a good simple blocking solution that can be used in a
>variety of applications (primary/secondary)? How about 1x PBS with 1% BSA?
> Many thanks.
> Glenn M. Krasinski
> San Diego, CA
> What are the most popular cars? Find out at Yahoo! Autos
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