RE: [Histonet] background in lymphoid tissue

From:"Patsy Ruegg"

It can be very difficult to quench endogenous peroxidase in some
tissue/cells, neutrophils in spleen and inflamation are notorious for being
difficult to quench.  If you can you might try using an alk.phos detection
system to see if it is the endogenous peroxidase giving you these problems.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
wk email
web site

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-----Original Message-----
[] On Behalf Of Johns, Laura
Sent: Friday, February 10, 2006 11:04 AM
To: ''
Subject: [Histonet] background in lymphoid tissue

Hi All,

I am having a problem with background staining in lymphoid tissue.  I have
tried increasing the time of my peroxide block, and adding in an
avidin/biotin blocking step, but still there is some slight staining in my
negative controls (no primary or secondary antibodies).  I have noticed this
in spleen and in other organs where there is an area of inflammation, and
with several different antibody combinations.  Has anyone experienced this
problem?  Any suggestions for getting rid of it?


Laura M. Johns, Ph.D.
Centocor, Inc.
145 King of Prussia Road (Mail Stop # R-4-2) Radnor, PA 19087
Phone:  610-240-8284 ....  Fax:  610-240-8150....  Email:

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