RE: [Histonet] Technovit 7100 resin removal

From:Pamela Marcum

Actually it will soften slightly and cause the GMA to curl and wrinkle 
badly however it will not remove it from the section.  It may lift it off 
the slide.  Unfortunately nothing has been found to successfully remove GMA=20
and in fact it continues to polymerize as it ages making it even more 
difficult to remove each day.  The polymerization even seems to continue on=20
the slides once the sections are picked up.  It has always seemed strange 
to me that Methoxide as we called it would remove epoxy and not GMA however=20
it is a fact.

Pam Marcum
UPENN Vet School

At 04:39 PM 2/7/2006, Rittman, Barry R wrote:
>Sodium methoxide has been used to remove epoxy resin, will this not work 
>on Technovit resin ?
>Barry
>
>-----Original Message-----
>From: histonet-bounces@lists.utsouthwestern.edu 
>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
>Sent: Tuesday, February 07, 2006 2:53 PM
>To: 'Gayle Callis'; germckeon@excite.com; Histonet@lists.utsouthwestern.edu
>Subject: RE: [Histonet] Technovit 7100 resin removal
>
>True Gayle, there are techniques to etch or soften the GMA but it cannot be
>dissolved.  We used to use a very strong ethoxide (alcohol in sodium
>hydroxide) solution to etch before staining (this method does not fair well
>for IHC).  As you mentioned, the best bet to do IHC is to use MMA.
>Patsy
>
>
>Patsy Ruegg, HT(ASCP)QIHC
>IHCtech, LLC
>Fitzsimmons BioScience Park
>12635 Montview Blvd. Suite 216
>Aurora, CO 80010
>P-720-859-4060
>F-720-859-4110
>wk email pruegg@ihctech.net
>web site www.ihctech.net
>
>
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>-----Original Message-----
>From: histonet-bounces@lists.utsouthwestern.edu
>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis
>Sent: Tuesday, February 07, 2006 1:37 PM
>To: germckeon@excite.com; Histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] Technovit 7100 resin removal
>
>Techovits 7100 is a Glycol methacrylate (GMA)  formulation and once
>polymerized, cannot be removed from the section or etched either.   Water
>may soften the plastic but it does NOT dissolve it away.   If you want to
>remove a plastic from a section for Immunostaining, then the Technovits
>9100, methyl methacrylate would be a better choice for embedding, then use
>60C xylene or a solvent that will dissolve the MMA away.
>
>    At 10:52 AM 2/7/2006, you wrote:
>
>
>
>
>
> >Hello Jorge,
> >
> >I am also trying to find out the same thing. Would it be possible to
> >send me any hints you may get?
> >
> >
> >
> >Many thanks,
> >
> >Gerald McKeon
> >
> >Trinity College,
> >
> >Dublin, Ireland.
> >
> >
> >
> >
> >
> >[Histonet] Technovit 7100 resin removal
> >
> >
> >
> >Hi all,
> >
> >
> >
> >I would like to remove the resin in my slides prior to stain. Do you
> >
> >know any protocol to do this?
> >
> >
> >
> >Thank you very much in advance
> >
> >
> >
> >Jorge Tornero
> >
> >IEO Cádiz - Spain
> >
> >
> >
> >_______________________________________________
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>
>Gayle Callis
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367
>406 994-4303 (FAX)
>
>
>
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Best Regards,

Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr.  CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348

Phone - 610-925-6278
Fax     - 610-925-8120
E-mail - pmarcum@vet.upenn.edu 



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