RE: [Histonet] Re: blue mucin

From:Kemlo Rogerson

Doesn't that just get round the problem of 'over-oxidised' stain? Is that
not just curing the symptoms but the problem remains that the haematoxylin
is over oxidised?

Kemlo Rogerson
Weston General Hospital
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194

-----Original Message-----
From: Tony Henwood [] 
Sent: Monday, February 13, 2006 10:07 PM
To: Michael LaFriniere;
Subject: RE: [Histonet] Re: blue mucin


The mucin staining is a function of the Hematoxylin pH. Lowering the pH
(usually by adding acetic acid) can significantly reduce mucin staining.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
[] On Behalf Of Michael
Sent: Tuesday, 14 February 2006 5:21 AM
Subject: [Histonet] Re: blue mucin

Any help would be appreciated....
I am noticing recently on routine H&E sections, the mucin on colon
biopsies only, demonstrating a strong blue color....I know the mucin is
picking up the hematoxylin stain and not differentiating in the acid
rinse. I was wondering if anybody has demonstrated this recently and the
possible corrections to end this aggravation. This leads me to believe
that it may be something with the patient prep prior to the biopsy? I am
using the Richard Allen (7221) Hematoxylin and routine reagents.  Any
suggestions would be greatly welcomed!
Thank you


Michael R. LaFriniere
Executive Director
Cytology Services of Maryland (CSM)
301-206-2555 ext 27
301-206-2595 fax


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