Michael,

The mucin staining is a function of the Hematoxylin pH. Lowering the pH
(usually by adding acetic acid) can significantly reduce mucin staining.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael
LaFriniere
Sent: Tuesday, 14 February 2006 5:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: blue mucin


Any help would be appreciated....
 
I am noticing recently on routine H&E sections, the mucin on colon
biopsies only, demonstrating a strong blue color....I know the mucin is
picking up the hematoxylin stain and not differentiating in the acid
rinse. I was wondering if anybody has demonstrated this recently and the
possible corrections to end this aggravation. This leads me to believe
that it may be something with the patient prep prior to the biopsy? I am
using the Richard Allen (7221) Hematoxylin and routine reagents.  Any
suggestions would be greatly welcomed!
 
Thank you
 

                                        


 
Michael R. LaFriniere
Executive Director
Cytology Services of Maryland (CSM)
301-206-2555 ext 27
301-206-2595 fax
michael.lafriniere@csmlab.com



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