RE: [Histonet] Iron demonstration in smears

From:Kemlo Rogerson

Obviously if they are to be 'fixed' you don't use a fixative containing
acid; if air dried, air dry then fix them in methanol (not Pap fix as it
contains glacial acetic acid) and in both cases they ought to last as long
as Pap smears, which I guess is months.

As long as the proteins are stabilised than everything ought to be Ok;
fungus however can be a problem as I've seen Pap smears infested with hyphae
after a few months. If you don't store them properly you can get such
contamination; interesting Carbo wax in the Pap Fix is supposed to act as a
barrier to many things; maybe you could take the acetic acid out?

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194

-----Original Message-----
From: Houston, Ronnie [] 
Sent: Monday, February 06, 2006 2:52 PM
To: Histonet (E-mail)
Subject: [Histonet] Iron demonstration in smears
Importance: High

One of our pathologists is asking how long can smears be left at room
temperature before being stained for hemosiderin; he is primarily concerned
with bone marrow and urine preps.

Ronnie Houston
Director of Anatomic Pathology
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 2877972
(804) 2877906 - fax




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