RE: [Histonet] Immunohistochemistry on mouse teeth

From:"Patsy Ruegg"

Have you decalcified the mouse teeth (I would imagine that you did)?  How
was the performed?  Decal can have a determental effect on IHC.  I use
formic acid for good IHC results.  If the calcium is not removed it can
block access to the antigen site.  Just some thoughts.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
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-----Original Message-----
[] On Behalf Of Amy Porter
Sent: Monday, February 06, 2006 12:25 PM
To: Horn, Diane A;
Subject: Re: [Histonet] Immunohistochemistry on mouse teeth

Diane - Are you using a mouse primary?  Didn't make a comment about this in
your original post.  If you are you would want to be using mouse on mouse
staining techniques or kits.  We always use another piece of tissue from the
same animal if possible as a postive control, usually something from G.I. 
tract.  Lots of proliferation going on there.  Hope this helps out,
----- Original Message -----
From: "Horn, Diane A" 
Sent: Sunday, February 05, 2006 11:19 PM
Subject: [Histonet] Immunohistochemistry on mouse teeth

I am currently doing IHC on develping mouse teeth and I am getting only
backgroung staining.  The tissues are fixed O/N in 4% paraformaldehyde @4
degrees and embedded in paraffin.  I have cut my sections @ 4um.  I have
tried HIER with Vector unmasking solution (boiling) for 2 minutes and using
a kit for PCNA from Zymed.  Has anyone done IHC on teeth that could give me
some advice on what to try next?


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