RE: [Histonet] A multi-use simple blocking solution?....

From:"Hermina Borgerink"

0.5% casein in your wash buffer and diluents.  Ref.: Tacha, D and
McKinney, L.  Casein Reduces Nonspecific Background staining in
Immunolabeling Techniques.  J Histotechnology 15:  2, June 1992

-----Original Message-----
[] On Behalf Of Gayle
Sent: Friday, February 10, 2006 10:46 AM
To: Glenn Krasinski;
Subject: Re: [Histonet] A multi-use simple blocking solution?....

We prefer to use normal serum blocking, with the serum matched to the
host of the secondary and do not use BSA based blockers.  This has been
discussed at great lengths on Histonet, check out Histonet Archives.
Blocking, per your question, is done many ways.

I suggest you do to the DAKO website, information and access the
Handbook of Immunochemical Methods, Boesnish discusses various blocking
agents, and you can download this wonderful freebie in pdf format.

You can buy superblocks ready to use from various companies, i.e.At
03:13 PM 2/9/2006, you wrote:
>I have been looking through the protocols on some of the IHC databases 
>for blocking solutions.  Some of the recipes are fairly exotic (fish 
>skin gelatin?).  Is there a good simple blocking solution that can be 
>used in a variety of applications (primary/secondary)?  How about 1x
PBS with 1% BSA?
>   Many thanks.
>   Glenn M. Krasinski
>   San Diego, CA
>  What are the most popular cars? Find out at Yahoo! Autos 
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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