[Histonet] water for frozen section staining

From:"Kapoor, Sue"

Hello histonetters,

We have a debate going in my lab as to using warm/hot or cold running water
when staining frozen sections.  One side states warm/hot water "blues" the
hematoxylin...the other sides feels the cold water keeps the sections from
falling off.

What does everyone in histo land do and think???

thanks for your replies :)

Sue Kapoor, HT (ASCP)
Histology Coordinator
Kenosha Medical Center
Kenosha, WI

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