[Histonet] plastic embedded bone sections
We routinely cut MMA embedded mouse and rat tibias using a 160 mm D-profile
tungsten carbide microtome blade. A good source of excellent
blades/resharpening service is Dorn/Hart microedge (Ken Hart), tel
#630-832-3843. Ken is also a great source of information as to the proper
blade angles, blades, etc for your application. I am not familiar with the
microm microtomes but we use a Leica 2165 motorized microtome (you
definitely need a motorized unit) and set our blade angle at approximately
2.5 to 3 (number setiing on side of knife holder). You may have to change
your angles as the blade is resharpened.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>