[Histonet] plastic embedded bone sections

From:"Michael J Voor"

Dear Histonet,

We are trying to section PMMA (Technovit 9100 New) embedded
undecalcified bone samples (rat tibias).  We plan to use a Microm
sliding microtome.  We need advice on exactly what blade to use and what
blade angle would be best to create sections on the order of 7-10
microns.  Thanks for any help.

Michael J. Voor, Ph.D.
Orthopaedic Bioengineering Laboratory
Director of Research
Department of Orthopaedic Surgery   
University of Louisville
Louisville, KY  40292
(502) 852-7673  FAX: (502)852-7227
e-mail: mike.voor@louisville.edu

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