Hello,
 
I normally perform DAB staining on free-floating brain sections in
staining nets from brain research labs.  However, I need to add an
antigen retrieval step at the beginning, 15 min in citrate buffer at
90C.  I've just tested the nets that I have, and they will not stand up
to the heat.  Does anyone know of a heat-resistant staining net?  Thank
you.
 
Caroline 
 
 
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