[Histonet] Re: overprocessing


Hi Kemlo,
I must disagree re hardening......if only in my experience of the following: 
I have processed gelatin ( 1%)blocks after formalin fixation (  1cm blocks 
fixed for 2, 5, 10 and 60 days) and find that they all suffer from shrinkage 
and hardening. My processing is via gradual alcohols to alc/xylene 1:1, then 
xylene x2 and wax x2. Two hrs in each.
Be grateful for enlightenment.
Best wishes

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