Calling all processing and reprocessing experts out there....
I'm sure all labs over the years have run into processing troubles ranging from instrument malfunctions during the night to misplacement of solutions (usually a water before the xylene), causing pure horror for the technologist who opens up the retort in the morning. It's sad to say that our department is experienced in these situations, and everytime something happens to the processing it's always difficult to decide how best to reprocess the sections.
Each time we've reprocessed our sections (usually due to the introduction of water before the xylene or paraffin stations) on shortened absolute alcohol, xylenes and paraffin times, they'v ended up being extremely hard and brittle, which does not make our pathologist happy....
Is there a way of reprocessing tissue without damaging it?
Would adding a softener to the alcohols reduce the hardening affects of reprocessing?
How much time can be safely shaved off if the tissues have already been through a regular program?
Any help is greatly appreciated,
Julia Celebre MLT
Hamilton General Hospital
905-527-0271 ext 46179
This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance.
Histonet mailing list
<< Previous Message | Next Message >>