To my knowledge you can't!!! Perhaps there is a solvent that does remove the resin but I'm affraid that it will also distroy your tissue. I have never read or heard of anyone who can. 
Try MMA (like Technovit 9100) instead of GMA (Technovit 7100).

léon Driessen
Orthopaedic Research Lab
UMC St. Radboud, Nijmegen
The Netherlands

-----Oorspronkelijk bericht-----
Van: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]Namens
histonet-request@lists.utsouthwestern.edu
Verzonden: dinsdag 7 februari 2006 19:22
Aan: histonet@lists.utsouthwestern.edu
Onderwerp: Histonet Digest, Vol 27, Issue 10


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Today's Topics:

   1. RE: Immunofluorescence Text (Houston, Ronnie)
   2. "MBS" in Luxol Fast Blue MBS (mlb@nmr.mgh.harvard.edu)
   3. Poster on RE: Color coding  (Gayle Callis)
   4. NEW TISSUE PROCESSOR (Sue Barnes)
   5. RE: "MBS" in Luxol Fast Blue MBS (Bartlett, Jeanine)
   6. small dryer (Steven Coakley)
   7. Re: small dryer (Maria Christensen)
   8. Re: Immunohistochemistry on mouse teeth (Amy Porter)
   9. RE: MSH6 (Barry Madigan)
  10. RE: Immunohistochemistry on mouse teeth (Patsy Ruegg)
  11. Re: "MBS" in Luxol Fast Blue MBS (John Kiernan)
  12. CPT codes for muscle biopsies (Garrison, Becky)
  13. Can anyone explain or at least empathise? (Cheryl)
  14. Can anyone explain or at least empathise? (Cheryl)
  15. Barcode Labeling on Tissue Slides (Gang Li)
  16. Kangaroo Mammary Tissue (Bruce Abaloz)
  17. RE: NEW TISSUE PROCESSOR (Anne Van Binsbergen)
  18. RE: Can anyone explain or at least empathise? (Kemlo Rogerson)
  19. Technovit 7100 resin removal (Jorge Tornero)
  20. troubles with CDK4 (jhaviland@mdanderson.org)
  21. Retic staining on rodent livers
      (Susan.Ferrigon@sanofi-aventis.com)
  22. Job posting : Sales and Support opportunities at	Improvision
      Inc (Pippa Simpson)
  23. decalcifying before HBQ stain (Julien Lambrey de Souza)
  24. JOB OPENING IN AUSTIN TEXAS (Chris Pomajzl)
  25. Luxol dyes (Robert Krug)
  26. re: decalcifying before HBQ stain (bhewlett@cogeco.ca)
  27. RE: Decalcifying before HBQ (Julien Lambrey de Souza)
  28. Technovit 7100 resin removal (germckeon@excite.com)


----------------------------------------------------------------------

Message: 1
Date: Mon, 6 Feb 2006 12:56:30 -0500 
From: "Houston, Ronnie" 
Subject: RE: [Histonet] Immunofluorescence Text
To: 'Patsy Ruegg' , 'David Haagsma'
	, histonet@lists.utsouthwestern.edu
Message-ID:
	<00FBB1F3374BE24AAB7ACC6FCC7C617F561F4C@bsrexms01.bshsir.com>
Content-Type: text/plain


the best text I have come across dealing with Immunofluorescence is:

Protein Localization by Fluorescence Microscopy: a practical approach
ed. VJ Allan
Oxford University Press 2000
ISBN 	0-19-963741-5 (hbk)
	0-19-963740-7 (pbk)



Ronnie Houston
Director of Anatomic Pathology
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 2877972
(804) 2877906 - fax
ronnie_houston@bshsi.com

  



-----Original Message-----
From: Patsy Ruegg [mailto:pruegg@ihctech.net]
Sent: Monday, February 06, 2006 12:51 PM
To: 'David Haagsma'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Immunofluorescence Text


The NSH IHC Resource Group has put together a list of reference books for
IHC.  I don't think there is specifically one on just F-IHC but several of
them relate.  You can join the IHCRG online at www.ihcrg.org for this and
many other benefits relating to IHC.
Patsy 


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg@ihctech.net
web site www.ihctech.net
 

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David
Haagsma
Sent: Monday, February 06, 2006 7:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Immunofluorescence Text

Dear Histonet;
 
We have a customer that is looking for a good immuno book. We currently do
not offer a book of this type and I do not have any recommendations for her
- can you help her out?
 
Thank you,
Dave Haagsma MT(ASCP)
MarketLab Inc.
 
 
 
 
Subject: Find Product Form
 
name...........:  Caroline Ghaleb
suggestion.....:  Dear Sir,
You have a good number of educational book, unfortunately I'm looking to buy
a book about immunofluorescence. I appreciate if you can let me know if you
can provide such a book.
 
Thanks a lot.
 
Caroline Ghaleb
phone..........:  
email..........:  teknolab@rcn.com
 
 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations.
In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information
contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s).  Should
this communication be received in error, please notify the sender immediately either by response e-mail or by phone,
and permanently delete the original e-mail, attachment(s), and any copies. 




------------------------------

Message: 2
Date: Mon, 6 Feb 2006 13:47:25 -0500 (EST)
From: mlb@nmr.mgh.harvard.edu
Subject: [Histonet] "MBS" in Luxol Fast Blue MBS
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<1146.132.183.203.6.1139251645.squirrel@mail.nmr.mgh.harvard.edu>
Content-Type: text/plain;charset=iso-8859-1

Hi, I had tried sending this message upon joining
histonet, but I didn't see it in the archive. That
could be why I haven't gotten a reply yet! Here it
is again:

Hi, I've been using luxol fast blue MBS for myelin
staining and would like to know what the acronym "MBS"
represents.

I have read many primary sources, such as articles by
Kluver and Barrera as well as Salthouse, and I have
performed internet searches with Google, all to no
avail (other than finding histonet!).

Thanks for your help!
Megan
=-)

p.s. Also, any explanations of the other acronyms used
     in luxol dyes (ARN, G and the "N" in MBSN) would
     be much appreciated!





------------------------------

Message: 3
Date: Mon, 06 Feb 2006 11:59:27 -0700
From: Gayle Callis 
Subject: [Histonet] Poster on RE: Color coding 
To: "Horn, Hazel V" ,
	Histonet@lists.utsouthwestern.edu
Message-ID:
	<6.0.0.22.1.20060206115815.01b4cab0@gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

Hazel,

I remember your excellent poster, it was very informative.


Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717




------------------------------

Message: 4
Date: Mon, 6 Feb 2006 14:34:53 -0500
From: "Sue Barnes" 
Subject: [Histonet] NEW TISSUE PROCESSOR
To: "Histonet \(E-mail\)" 
Message-ID: 
Content-Type: text/plain;	charset="iso-8859-1"

We are in the market for a new tissue processor.  I am looking for one that is compatable with recycling of alcohol.  Could I please hear from those of you who recycle alcohol and how well your processor works for you.

Susan Barnes
East Liverpool City Hospital
East Liverpool, Ohio 


------------------------------

Message: 5
Date: Mon, 6 Feb 2006 14:42:53 -0500
From: "Bartlett, Jeanine" 
Subject: RE: [Histonet] "MBS" in Luxol Fast Blue MBS
To: ,	
Message-ID:
	
Content-Type: text/plain;	charset="US-ASCII"

Luxol fast blue MBSN is the chemical description for the specific dye,
Solvent Blue 38 
C32H12CuN8Na2O6S2 
F.W. 732.16 


Jeanine Bartlett, BS, HT(ASCP)
Centers for Disease Control and Prevention
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
mlb@nmr.mgh.harvard.edu
Sent: Monday, February 06, 2006 1:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] "MBS" in Luxol Fast Blue MBS

Hi, I had tried sending this message upon joining histonet, but I didn't
see it in the archive. That could be why I haven't gotten a reply yet!
Here it is again:

Hi, I've been using luxol fast blue MBS for myelin staining and would
like to know what the acronym "MBS"
represents.

I have read many primary sources, such as articles by Kluver and Barrera
as well as Salthouse, and I have performed internet searches with
Google, all to no avail (other than finding histonet!).

Thanks for your help!
Megan
=-)

p.s. Also, any explanations of the other acronyms used
     in luxol dyes (ARN, G and the "N" in MBSN) would
     be much appreciated!



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 6
Date: Mon, 6 Feb 2006 12:04:07 -0800 (PST)
From: Steven Coakley 
Subject: [Histonet] small dryer
To: Histonet@lists.utsouthwestern.edu
Message-ID: <20060206200407.41079.qmail@web90210.mail.scd.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Does anyone know where I might be able to get a small forced air dryer, holding 2-3 racks of slides?
   
  Steve

		
---------------------------------
Brings words and photos together (easily) with
 PhotoMail  - it's free and works with Yahoo! Mail.

------------------------------

Message: 7
Date: Mon, 6 Feb 2006 15:01:37 -0600
From: Maria Christensen 
Subject: Re: [Histonet] small dryer
To: Steven Coakley ,
	
Message-ID: 
Content-Type: text/plain; charset="iso-8859-1" ; format="flowed"

>Does anyone know where I might be able to get a 
>small forced air dryer, holding 2-3 racks of 
>slides?
>   
>   Steve
>
>
>---------------------------------
>Brings words and photos together (easily) with
>  PhotoMail  - it's free and works with Yahoo! Mail.
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

We have one from TBS, Slide Dryer II.  It works 
pretty well for what we use it (dry 130-160 
slides O.N. at 37C) but it can dry slides in 
about half an hour.  I think it lists for $1500 
at Fisher Scientific, but we get a nice discount 
from them.

Hope this helps!
-- 
María

Maria A. Christensen
Technical Associate
Dept. of Medical Microbiology & Immunology
Creighton University School of Medicine
2500 California Plaza
Omaha, Nebraska  68178-0404

voice	(402) 280-2678
e-mail	mariac@creighton.edu



------------------------------

Message: 8
Date: Mon, 6 Feb 2006 14:25:02 -0500
From: "Amy Porter" 
Subject: Re: [Histonet] Immunohistochemistry on mouse teeth
To: "Horn, Diane A" ,
	
Message-ID: <001001c62b53$08344100$8e7a0923@HistoJJ>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

Diane - Are you using a mouse primary?  Didn't make a comment about this in 
your original post.  If you are you would want to be using mouse on mouse 
staining techniques or kits.  We always use another piece of tissue from the 
same animal if possible as a postive control, usually something from G.I. 
tract.  Lots of proliferation going on there.  Hope this helps out,
----- Original Message ----- 
From: "Horn, Diane A" 
To: 
Sent: Sunday, February 05, 2006 11:19 PM
Subject: [Histonet] Immunohistochemistry on mouse teeth



I am currently doing IHC on develping mouse teeth and I am getting only 
backgroung staining.  The tissues are fixed O/N in 4% paraformaldehyde @4 
degrees and embedded in paraffin.  I have cut my sections @ 4um.  I have 
tried HIER with Vector unmasking solution (boiling) for 2 minutes and using 
a kit for PCNA from Zymed.  Has anyone done IHC on teeth that could give me 
some advice on what to try next?

Thanks!
Diane


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 9
Date: Tue, 7 Feb 2006 07:51:24 +1000
From: "Barry Madigan" 
Subject: RE: [Histonet] MSH6
To: 
Message-ID: <000001c62b67$7a6eb7a0$0201010a@WORKSTATION1>
Content-Type: text/plain;	charset="us-ascii"

Hello Josie,
 
The antibodies for mismatch repair genes that we use consists of MLH1, MSH2
and MSH6 all of which we obtain from BD (Becton Dickinson).
They are all performed on our BOND MAX Immunostainer using the high pH
retrieval solution with EDTA (30 min) and a Vision Polymer Detection System.
We have no troubles with them except sometimes we find that with archival
cases we need to apply extra heat for MLH1.
When this extra step is performed the MLH1 internal controls come up
beautifully.
Before we had the BOND MAX we used to heat retrieve using a microwave
pressure cooker for 8 minutes on high in TRIS/EDTA pH 9.0 for MLH1. 
 
For the MSH6 antibody we currently obtain a dilution of 1:200.
MSH2 1:500 and MLH1 1:100.
 
Hope this is of some help.
Regards
Barry 
 
Barry Madigan
Immunohistochemistry
QHPS-Royal Brisbane Hospital Campus
Australia
 
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca
Sent: Saturday, 4 February 2006 1:24 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] MSH6
 
 
Hi Josie,
How many different types of protocols did you try on the XT?
With the different temp and Cell conditioning options I'm surprised you
didn't get something.
Are you running the MLH-1 and MSH-2 as well?  How are they?
 
My experience with the Microsateliite Instabilities is 4+ staining in 2
out of 3 antibodies, depending on the control tissue.  The third
antibody would compare at 2+ or a bit stronger.
 
I have the best of both worlds with a Benchmark and a Nemesis.  I
experienced the poor staining results of these particular antibodies and
I decided to run them on the Nemesis with Biocare polymer detection and
they are gorgeous. I use the Biocare antibodies, too.
In case you don't have that option, I would consider trying another
control tissue, and running through all the CC options.   I'm not sure
if they behave differently without heat, did you try running a protocol
with out heat in the detection? 
Do you have access to the polymer from Ventana?  Maybe try that?  Or run
the antibody at 1:5 or 1:10.
Or do you have a pressure cooker to try the HEIR offline.  If you get
good staining with offline HEIR then you can rule out the detection as
the issue, too.
 
Becky
 
 Becky Orr CLA,HT(ASCP)
IHC Lead 
Evanston Northwestern Healthcare
847-570-2771
 
-----Original Message-----
 
 
Message: 8
Date: Wed, 1 Feb 2006 13:54:36 -0600
From: "Nava, Josefa" 
Subject: [Histonet] looking for Antibodies-MSH6  and PMS2
To: 
Message-ID:
      <2C515C1049EAF5459EFD8C9B929078A41945A8@phdex03.txhealth.org>
Content-Type: text/plain;     charset="us-ascii"
 
Hello Everyone ,
I am using Ventana BMK/XT , can someone tell me a good source of MSH6
and PMS2  that will work  on the Ventana  Machine. I  appreciate  any
information. Thank you.
 
Josie 
 
 
 
 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 10
Date: Mon, 6 Feb 2006 15:05:30 -0700
From: "Patsy Ruegg" 
Subject: RE: [Histonet] Immunohistochemistry on mouse teeth
To: "'Amy Porter'" , "'Horn, Diane A'"
	,	
Message-ID: <200602062205.k16M5NDZ009781@chip.viawest.net>
Content-Type: text/plain;	charset="US-ASCII"

Have you decalcified the mouse teeth (I would imagine that you did)?  How
was the performed?  Decal can have a determental effect on IHC.  I use
formic acid for good IHC results.  If the calcium is not removed it can
block access to the antigen site.  Just some thoughts.
Patsy 


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg@ihctech.net
web site www.ihctech.net
 

This email is confidential and intended solely for the use of the Person(s)
('the intended recipient') to whom it was addressed. Any views or opinions
presented are solely those of the author. It may contain information that is
privileged & confidential within the meaning of applicable law. Accordingly
any dissemination, distribution, copying, or other use of this message, or
any of its contents, by any person other than the intended recipient may
constitute a breach of civil or criminal law and is strictly prohibited. If
you are NOT the intended recipient please contact the sender and dispose of
this e-mail as soon as possible.


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Porter
Sent: Monday, February 06, 2006 12:25 PM
To: Horn, Diane A; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Immunohistochemistry on mouse teeth

Diane - Are you using a mouse primary?  Didn't make a comment about this in
your original post.  If you are you would want to be using mouse on mouse
staining techniques or kits.  We always use another piece of tissue from the
same animal if possible as a postive control, usually something from G.I. 
tract.  Lots of proliferation going on there.  Hope this helps out,
----- Original Message -----
From: "Horn, Diane A" 
To: 
Sent: Sunday, February 05, 2006 11:19 PM
Subject: [Histonet] Immunohistochemistry on mouse teeth



I am currently doing IHC on develping mouse teeth and I am getting only
backgroung staining.  The tissues are fixed O/N in 4% paraformaldehyde @4
degrees and embedded in paraffin.  I have cut my sections @ 4um.  I have
tried HIER with Vector unmasking solution (boiling) for 2 minutes and using
a kit for PCNA from Zymed.  Has anyone done IHC on teeth that could give me
some advice on what to try next?

Thanks!
Diane


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 11
Date: Mon, 06 Feb 2006 17:12:53 -0500
From: John Kiernan 
Subject: Re: [Histonet] "MBS" in Luxol Fast Blue MBS
To: mlb@nmr.mgh.harvard.edu
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <43E7C9E5.B2FC7F08@uwo.ca>
Content-Type: text/plain; charset=us-ascii

Your message appeared all right, but nobody
answered. It probably means nobody knows what the
MBS stands for. Luxol fast blues are not a
chemical group of dyes. They are arylguanidinium
salts of anionic dyes (rather than the usual
sodium salts). This makes the dyes soluble in
alcohol but not in water. When sections are
stained, the organic cation stays in the alcohol,
and the coloured anion lodges in the rather
myelin. Like any other anionic dye, a luxol can be
differentiated by alkali. Luxol fast blue MBS is a
phthalocyanine dye; luxol fast blues G and ARN are
azo dyes. See Clasen et al. 1973 J. Neuropath.
Exp. Neurol. 32:271-283 for more on this subject,
including how to make your own luxol-type dyes.

John Kiernan
London, Canada
-----------------------------
mlb@nmr.mgh.harvard.edu wrote:
> 
> Hi, I had tried sending this message upon joining
> histonet, but I didn't see it in the archive. That
> could be why I haven't gotten a reply yet! Here it
> is again:
> 
> Hi, I've been using luxol fast blue MBS for myelin
> staining and would like to know what the acronym "MBS"
> represents.
> 
> I have read many primary sources, such as articles by
> Kluver and Barrera as well as Salthouse, and I have
> performed internet searches with Google, all to no
> avail (other than finding histonet!).
> 
> Thanks for your help!
> Megan
> =-)
> 
> p.s. Also, any explanations of the other acronyms used
>      in luxol dyes (ARN, G and the "N" in MBSN) would
>      be much appreciated!
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 12
Date: Mon, 6 Feb 2006 17:14:00 -0500
From: "Garrison, Becky" 
Subject: [Histonet] CPT codes for muscle biopsies
To: 
Message-ID:
	
Content-Type: text/plain;	charset="iso-8859-1"

Could any of you share the CPT codes used for muscle biopsy work ups?
 My question is in 2 parts:
1) We currently charge:
	88314 per stain for  ATP, NADH, Esterase, Alkaline Phosphatase,
	          Acid Phosphatase, AMPDA, Myophorylase, Cyto C Oxidase, and SDH
	          on snap frozen muscle tissue

   and      88313  per stain for Trichrome, PAS, Oil Red O (ORO) on snap frozen muscle tissue

   in addition to 88305 for the biopsy itself.

One pathologist is suggesting that we should be charging 88319 for the ATP thru SDH above
and 88314 for the Trichrome thru ORO above and that 88313 to be used  only on Trichromes, 
PAS and ORO on paraffin embedded tissue (such as for the nerve biopsies).  

In reviewing the 2006 CPT code book, it does seem that we may be charging incorrectly.  

2) If 88319 is the correct answer to the first part:  88319 is not listed as an add on code.   
    Do you still charge an 88305 for the biopsy?

How would you charge a muscle biospsy, received fresh, divided into 2 parts as follows:
First part is snap frozen in isopentane; frozen sections are stained with ATP x 3, SDH, Trichrome, 
ORO.
Second part is submitted for routine processing and paraffin sections are stained with H&E, Trichrome 
and ORO?


Thanks for your input.

Becky Garrison
Pathology Supervisor
Jacksonville, FL  
904-244-6237





   









------------------------------

Message: 13
Date: Mon, 6 Feb 2006 16:01:57 -0800 (PST)
From: Cheryl 
Subject: [Histonet] Can anyone explain or at least empathise?
To: histonet@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <20060207000157.95181.qmail@web50913.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hey Fellow Histonetters:
  I read all the postings on here and generally understand all of it, but the more I read the more I realize I've forgotten more than I probably remember. (I haven't done a muscle stain group in quite a while!!).  Does anyone else feel this way???  (Yes, I've passed 40, I'm sure someone out there can explain the mechanism of memory loss for me!!!)
   
  Thanks for slowing down the brain drain!!
   
  Cheryl Kerry, HT(ASCP)
  Full Staff Inc.
  Staffing the AP Lab by helping one tech at a time.
  admin@fullstaff.org
  281.852.9457 office
  281.883.7704 cell

 


------------------------------

Message: 14
Date: Mon, 6 Feb 2006 16:01:57 -0800 (PST)
From: Cheryl 
Subject: [Histonet] Can anyone explain or at least empathise?
To: histonet@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <20060207000157.95181.qmail@web50913.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hey Fellow Histonetters:
  I read all the postings on here and generally understand all of it, but the more I read the more I realize I've forgotten more than I probably remember. (I haven't done a muscle stain group in quite a while!!).  Does anyone else feel this way???  (Yes, I've passed 40, I'm sure someone out there can explain the mechanism of memory loss for me!!!)
   
  Thanks for slowing down the brain drain!!
   
  Cheryl Kerry, HT(ASCP)
  Full Staff Inc.
  Staffing the AP Lab by helping one tech at a time.
  admin@fullstaff.org
  281.852.9457 office
  281.883.7704 cell

 


------------------------------

Message: 15
Date: Mon, 6 Feb 2006 19:36:09 -0500
From: Gang Li 
Subject: [Histonet] Barcode Labeling on Tissue Slides
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<120b7bf0602061636l18f78fb7m315a28e5c321e5c6@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Could anybody kindly tell me the barcode standards used for tissue slides
labeling?



Best wishes



Gang Li, Ph.D.

Biomedical Photometrics Inc.

Waterloo, Ontario, CANADA


------------------------------

Message: 16
Date: Tue, 07 Feb 2006 13:14:52 +1100
From: Bruce Abaloz 
Subject: [Histonet] Kangaroo Mammary Tissue
To: histoNet@Pathology.swmed.edu
Message-ID: 
Content-Type: text/plain; charset="us-ascii" ; format="flowed"

Dear Histonetter's,
I am looking for stains that will identify the basic contents of 
cultured, mammary alveoli (mammospheres) of Kangaroo. Broad lipid ( 
ANY FAT STAIN THAT CAN BE DONE ON PARAFFIN EMBEDDED 
TISSUE???POSSIBLE??), carbohydrate (PAS?? ANY OTHER SUGGESTIONS) and 
protein stains (???) are required to determine what - IF any, 
different hormone combinations have/ effect on milk production.
Any suggestions would be wonderful......our Histologist has made 
several good suggestions for me to try & we are both interested to 
see 'WHAT' POSSIBILITIES/suggestions the scientific minds in 
Histo-land come up with.
  Thankyou in advance & Cheers,
Sonia

-- 
  BRUCE ABALOZ 
PH:61383446282
  HISTOLOGIST 
FAX:61383447909
  DEPT.of ZOOLOGY 
EMAIL: brucea@unimelb.edu.au
  THE UNIVERSITY Of MELBOURNE.  VICTORIA. AUSTRALIA 3010          
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astronaut. 
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------------------------------

Message: 17
Date: Tue, 7 Feb 2006 08:11:12 +0400
From: "Anne Van Binsbergen" 
Subject: RE: [Histonet] NEW TISSUE PROCESSOR
To: "Sue Barnes" ,	"Histonet (E-mail)"
	
Message-ID:
	<7E070A7B959A9F42BE732545E5CF6210948D4D@SKMCEMAIL.skmc.gov.ae>
Content-Type: text/plain;	charset="us-ascii"

We use recycled alcohol and have a Sakura VIP5 - in my opinion they are
the best - no problems whatsoever. Recycled alcohol should not make any
difference.
Annieinarabia

-----Original Message-----
From: Sue Barnes [mailto:SBarnes@elch.org] 
Sent: Monday, February 06, 2006 11:35 PM
To: Histonet (E-mail)
Subject: [Histonet] NEW TISSUE PROCESSOR

We are in the market for a new tissue processor.  I am looking for one
that is compatable with recycling of alcohol.  Could I please hear from
those of you who recycle alcohol and how well your processor works for
you.

Susan Barnes
East Liverpool City Hospital
East Liverpool, Ohio 
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------------------------------

Message: 18
Date: Tue, 7 Feb 2006 08:48:32 -0000 
From: Kemlo Rogerson 
Subject: RE: [Histonet] Can anyone explain or at least empathise?
To: "'Cheryl'" ,
	histonet@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain

I've rationalised from the following standpoint;

When you are younger you usually have less to think about and therefore you
can focus your thoughts better. As you get older you have more to think
about and usually less time and your thoughts become unfocused. I mean my
son arranged perfectly easily to organise his snow boarding holiday but
fails regularly to pay his rent. 

I think when you get older you also care more about forgetting; damn where
are my glasses!!!

Kemlo Rogerson
Pathology Manager
Ext  3311
DD   01934 647057
Mob 07749 754194
 
 
 

-----Original Message-----
From: Cheryl [mailto:tkngflght@yahoo.com] 
Sent: Tuesday, February 07, 2006 12:02 AM
To: histonet@lists.utsouthwestern.edu
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Can anyone explain or at least empathise?

Hey Fellow Histonetters:
  I read all the postings on here and generally understand all of it, but
the more I read the more I realize I've forgotten more than I probably
remember. (I haven't done a muscle stain group in quite a while!!).  Does
anyone else feel this way???  (Yes, I've passed 40, I'm sure someone out
there can explain the mechanism of memory loss for me!!!)
   
  Thanks for slowing down the brain drain!!
   
  Cheryl Kerry, HT(ASCP)
  Full Staff Inc.
  Staffing the AP Lab by helping one tech at a time.
  admin@fullstaff.org
  281.852.9457 office
  281.883.7704 cell

 
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------------------------------

Message: 19
Date: Tue, 7 Feb 2006 13:32:52 +0100
From: Jorge Tornero 
Subject: [Histonet] Technovit 7100 resin removal
To: histonet@lists.utsouthwestern.edu
Message-ID: <8c964a790602070432r457655a2x@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi all,

I would like to remove the resin in my slides prior to stain. Do you
know any protocol to do this?

Thank you very much in advance

Jorge Tornero
IEO Cádiz - Spain



------------------------------

Message: 20
Date: Tue, 7 Feb 2006 08:43:35 -0600
From: jhaviland@mdanderson.org
Subject: [Histonet] troubles with CDK4
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
	
Content-Type: text/plain; charset=us-ascii

Hello:
I am trying to do IHC with rabbit anti human CDK4 (D-22) from Santa Cruz on
salivary gland tissue.  I am having background problems which is leading to
difficulty in figuring out what is positive and what is negative.  I have
used LSAB2 kit from Dako,  Rabbit Envision Polymer from Dako and the Rabbit
ABC Elite kit from Vector. I do a 3%  hydrogen peroxide treatment for 20
minutes and do antigen retrieval with 10mM Citric Buffer pH 6.0 for 45
minutes in a steamer and a 20 minute cool down.  I do a blocking step and
have used either serum free or goat serum for a block.

I already checked with the company about my protocol and it is the same as
theirs.  I tried doing it on skin which is reccommended as a control but
still have background problems.

Thanks
Joie Haviland
Senior Research Assistant
MD Anderson Cancer Research Center






------------------------------

Message: 21
Date: Tue, 7 Feb 2006 14:53:48 +0000
From: Susan.Ferrigon@sanofi-aventis.com
Subject: [Histonet] Retic staining on rodent livers
To: histonet@lists.utsouthwestern.edu,
	histonet-bounces@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset=ISO-8859-1





Does anyone have problems with staining for reticulin on rodent livers??
if not, do you have a particular method you use??
I use Gordon and Sweet and find the fibres don't stain up very well and the
background is grainy.  Any advise.

Thanks
Susan
------------------------------
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------------------------------




------------------------------

Message: 22
Date: Tue, 7 Feb 2006 15:04:07 -0000
From: "Pippa Simpson" 
Subject: [Histonet] Job posting : Sales and Support opportunities at
	Improvision Inc
To: 
Message-ID: <017d01c62bf7$bedc6870$0701a8c0@improvision.com>
Content-Type: text/plain;	charset="iso-8859-1"

Please forgive the commercial intrusion.  Improvision is currently considering applicants for roles within our Sales and Technical Support teams. For further details please visit http://www.improvision.com/careers/ or contact recruitment@improvision.com.  Please do not reply to the listserver.

Kind regards

Pippa Simpson

Improvision Human Resources


------------------------------

Message: 23
Date: Tue, 7 Feb 2006 10:50:25 -0500
From: Julien Lambrey de Souza 
Subject: [Histonet] decalcifying before HBQ stain
To: histonet@lists.utsouthwestern.edu
Message-ID: <75c6cee121c47a552bf01aa9b6910f1b@uqar.qc.ca>
Content-Type: text/plain; charset=US-ASCII; format=flowed

Hello everyone,

Just a quick question: Is it necessary to decalcify before staining 
with the HBQ protocol?

Thanks,


Julien Lambrey de Souza
Research assistant, Evolutionary biology
University of Quebec at Rimouski

Tel: (418) 723-1986 #1714
Fax: (418) 724-1849




------------------------------

Message: 24
Date: Tue, 7 Feb 2006 10:12:11 -0600
From: "Chris Pomajzl" 
Subject: [Histonet] JOB OPENING IN AUSTIN TEXAS
To: 
Message-ID: <006901c62c01$43393590$26fca8c0@CSP>
Content-Type: text/plain;	charset="iso-8859-1"

Greetings:

We have multiple shift openings in Austin, Texas. If you might be interested in a fast-paced, high-volume laboratory, please give me a call. Duties include embedding, sectioning, special stains, etc. Competetive salary, health benefits, and a friendly working environment. Thanks.

Sincerely,

Chris Pomajzl, HTL (ASCP)

Histology Supervisor
Clinical Pathology Laboratories, Inc.
512.873.1660 (o)
512.873.5004 (f)
cpomajzl@cpllabs.com

CONFIDENTIALITY NOTICE:  This communication is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law.  If you are not the intended recipient, you are notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy this communication.

------------------------------

Message: 25
Date: Tue, 7 Feb 2006 10:17:43 -0600
From: Robert Krug 
Subject: [Histonet] Luxol dyes
To: histonet@lists.utsouthwestern.edu
Cc: mlb@nmr.harvard.edu
Message-ID:
	
Content-Type: text/plain; charset="US-ASCII"

Megan: 

Saw  Dr Kiernan's posting on Histonet.  I cannot tell you what the MBS 
stands for, but I can add a little insight into naming dyes.  Dyes are 
given trivial names in most cases.   This really means the person 
originating the dye may call the dye whatever they please.  At one time 
there were may have existed guidelines.  If such guidelines existed, no 
one seems to be familiar with the specifics at this late date.   Today we 
use scientific nomenclature with set conventions to name products such as 
1-Cyclohexyl-2-phyrrolidone.  Personally I prefer the trivial names.

In some cases the abbreviations come from the German dye industry - who 
were really pioneers in the field.

The SF in Light Green SF yellowish = SaureFarbstoff (German) = acid dye

English terms are also sometimes abbreviated

FCF in Fast Green FCF = For Coloring Food

There are a series of fluorescent dyes available such as PKH26, PKH67.  In 
this particular case I cannot be 100% sure, but the person who filed the 
patent on these dyes just happened to have the initials PKH.  Coincidence? 
 In this particular case I would guess that the 26th and the 67th attempts 
were keepers.

Naphthol compounds are often named Naphthol AS-MX phosphate or possibly 
Naphthol AS-BI phosphate.  The structures are evidently different, but no 
one I have talked to has been able to explain why one product is given as 
the AS-MX designation and the next product is AS-BI

If you look at Conn's Biological Stains, 9th Edition, you will find the 
text lists many more obscure synonyms - as compared to the 10th Edition. 
In previous years, companies often gave common dyes unique names.  They 
may have believed they gained some competitive advantage from this 
practice. Even today dyes may often go by more than one name.  Sometime 
this is done when the company markets to various industries.  If you are 
selling to the printing industry, you might name a dye XXXX.  If you 
market dyes for histological use, you might name a dye YYYY.  Today I 
believe the trend is for companies to sell the dye by the name given the 
most common product of commerce.  This is probably why fewer synonyms are 
listed in the 10th Edition. Synonyms however are here to stay.  Sodium 
thiosulfate is still referred to as hypo in older textbooks.  This is 
because "hypo" was the term used in photography.

One of the many useful functions served by the Biological Stain Commission 
was the creation of CI or Color Index numbers.  It really doesn't matter 
what a company names a dye.  If Product X and Product Y from competing 
companies have the same CI number, there is a good chance the dyes may be 
used in the same staining procedures.  If the dyes are Certified by the 
BSC, this gives even more indication the dye should be acceptable for use. 
 The CI number varies from the CAS number.  For the CAS numbers to be 
identical, the molecular structure should be identical.  Not so with Color 
Index numbers.

For example, Basic Fuchsin may be composed of 100% Pararosaniline, or a 
mixture of pararosaniline, rosaniline, magenta II and magenta III. The 
pararosaniline may be the chloride or the acetate form.   So although the 
blends may vary from manufacturer to manufacturer and possibly even 
between lot numbers for a specific manufacturer, the dye must perform in 
an acceptable manner to receive certification from the Biological Stain 
Commission. In the 10th Edition of Conn's, no CI number is assigned to 
Basic Fuchsin, although the individual homologs are given given unique 
homologs. The 9th Edition of Conn's assigned Basic Fuchsin the CI number 
for Pararosanline. The real test is how well the dyes perform in actual 
staining procedures. Anyone really interested in dyes for biological use 
should try to obtain a copy of the methods used by the Biological Stain 
Commission.  Analysis and testing of biological stains - The Biological 
Stain Commission Procedures  (Biotechnic & Histochemistry 2002, 77(5&6): 
237-275

Methylene Blue is another prime example for a dye which is composed of a 
series of closely related homologs.  However in this case Methylene Blue 
is assigned a Color Index number, as opposed to Basic Fuchsin which was 
not assigned a unique CI number in the 10th Edition of Conn's. Again the 
major homologs are assigned CI numbers.

Other dyes by comparison are relatively pure and may be almost 100% pure.  
However sometimes purity is not all.  Some of the impurities may be 
present/added/act as stabilizers. In the case of Nile Blue, the presence 
of Nile Red as a contaminant may be highly desirable.

For other dyes the formulations are constantly shifting, even though the 
name remains relatively constant.  Alcian Blue is a dye which has a 
tendency to explode during manufacture.  This has resulted in a constant 
tinkering with the method of production over the years.  The dye sold 
today was probably manufactured very differently than an Alcian Blue 
manufactured decades ago.  If the product of commerce continues to provide 
acceptable staining results in the methods commonly associated with Alcian 
Blue, the dye will be sold & certified as Alcian Blue. It would be 
impractical to give each slight variation in product a unique name or CAS 
number. Trying to perform a literature search would be made extremely 
difficult to almost impossible. 

Tradition methyl green has not been produced commercially for many years 
(35-45+ ?). The actual product of commerce today is ethyl green.  In this 
case methyl green and ethyl green are really not synonyms.   However 
because ethyl green may be used for the same purposes as methyl green, the 
product of commerce continues to be called methyl green - very much to the 
annoyance of Dr Kiernan :)

Hope this helps,

Best Regards,

Bob Krug
Sigma-Aldrich
St Louis, MO








------------------------------

Message: 26
Date: Tue, 07 Feb 2006 11:37:48 -0500
From: bhewlett@cogeco.ca
Subject: re: [Histonet] decalcifying before HBQ stain
To: Julien Lambrey de Souza ,
	histonet@lists.utsouthwestern.edu
Message-ID: <43e8ccdc.319.3f3d.9906@cogeco.ca>

> Julien,
Just what is the HBQ protocol?

Bryan

> Hello everyone,
>
> Just a quick question: Is it necessary to decalcify before staining
> with the HBQ protocol?
>
> Thanks,
>
>
> Julien Lambrey de Souza
> Research assistant, Evolutionary biology
> University of Quebec at Rimouski
>
> Tel: (418) 723-1986 #1714
> Fax: (418) 724-1849
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 27
Date: Tue, 7 Feb 2006 12:50:31 -0500
From: Julien Lambrey de Souza 
Subject: [Histonet] RE: Decalcifying before HBQ
To: histonet@lists.utsouthwestern.edu
Message-ID: <634240d185312d7b6108d64157d0b1e0@uqar.qc.ca>
Content-Type: text/plain; charset=US-ASCII; format=flowed

Sorry. I guess I should have elaborated a bit on the stain.

HBQ = Hall and Brunt's Quadruple stain
Used to differenciate bone from cartillage and chondroid bone.

Deparaffinized sections are stained in aqueous celestine blue (0,5% in 
5% ammonium sulphate and 14% glycerin), Mayer's hematox, alcian blue 
(1% in 1% acetic acid, pH 2,6), Phosphomolybdic acid1% and aqueous 
direct red 0,5%.

All the protocols and pictures I have seen in publications are done on 
decalcified tissue. My question is the following: If my specimen cuts 
out nice sections without decalcification, will I get the intended 
staining (same as seen in publications) or will it be different from 
decalcified sections?

How does decalcification influence staining results?

Thanks for the imput.


Julien Lambrey de Souza
Research assistant, Evolutionary biology
University of Quebec at Rimouski

Tel: (418) 723-1986 #1714
Fax: (418) 724-1849




------------------------------

Message: 28
Date: Tue,  7 Feb 2006 12:52:02 -0500 (EST)
From: "germckeon@excite.com" 
Subject: [Histonet] Technovit 7100 resin removal
To: jorge.tornero@gmail.com
Cc: histonet@lists.utsouthwestern.edu
Message-ID: <20060207175202.C3E6137628@xprdmailfe14.nwk.excite.com>
Content-Type: text/plain; charset="us-ascii"






Hello Jorge,

I am also trying to find out the same thing. Would it be possible to send me any hints you may get?



Many thanks,

Gerald McKeon

Trinity College, 

Dublin, Ireland.





[Histonet] Technovit 7100 resin removal



Hi all,



I would like to remove the resin in my slides prior to stain. Do you

know any protocol to do this?



Thank you very much in advance



Jorge Tornero

IEO Cádiz - Spain



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End of Histonet Digest, Vol 27, Issue 10
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