[Histonet] RE: Pre-treatment module for immunostainers

From:"Promila Rastogi"

FYI, BioGenex has a pretreatment system called the EZ Retriever. It is a temperature and time controlled microwave based system and can treat up to 96 slides in one run (each run is no more than 10 minutes). You may use your own Antigen Retrieval solutions (which are aqueous), or BioGenex's. Decide what temperature you want to treat your slides at. 

Promila Rastogi,
Product Manager
4600 Norris Canyon Road
San Ramon, CA 94583
Tel: 925.866-2577
Fax: 925.866-2594

Message: 19
Date: Fri, 17 Feb 2006 09:49:57 -0800 (PST)
From: Rene J Buesa 
Subject: Re: [Histonet] Pre-treatment module for immunostainers
To: meint002 , histonet@lists.utsouthwestern.edu
Message-ID: <20060217174957.75639.qmail@web61211.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Dear Joyce:
  Unless you are short in trained personnel and perform more than 100 IHC slides daily, I personally do not see the "advantage" of limiting yourself to a company that will sell you proprietary (and expensive) reagents that will lead you to a "closed system".
  "Ventana" has a new IHC automatic instrument that also deparafinizes and performs HIER and I have heard both good and bad things about it.
  I always used Dako and laboratories that I have consulted for also use Dako (up to 3 autostainers) and the "beautity" of the Dako instrument is that it is an "open" technology that you can use even with home made reagents (HIER buffers, Ab diluents) as well as any Ab from any manufacturer.
  To dewax your slides will take less than 30 minutes (and it can be done with your regular H&E autostainer) and HIER requires, at the most, 1 hour, and you don't have to stand by during that time.
  I would not commit myself to a single manufacturer product.
  I hope that this will help you!
  René J. 

meint002  wrote:
  Dear Histos,

I am in the process of determining which immunostainer to purchase for our
lab. DAKO and Lab Vision are the main vendors at the moment. Lab Vision has
a new device that they claim will automate the dewaxing and epitope recovery
on your slide before you apply the antibodies. 

Does anyone have experience with this Pre-treatment module or a device of a
similar nature? If so, do they work very well, or are they worth the
expense? I think you can use your own reagents for the various buffers - so
that could keep the reagent cost down some. Otherwise, they would be happy
to sell you their own products.

Any advice on this matter would be greatly appreciated. 
Thank you in advance for your feedback.

Joyce Meints

University of Minnesota
Paul and Sheila Wellstone Muscular Dystrophy Center
MMC 206
420 Washington St. SE
Minneapolis, MN 55455-0392

e-mail: meint002@umn.edu
lab phone: 612-626-4703

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