[Histonet] RE: Ihc bone staining

From:"Allen, Rhonda"

> Does anyone know if it could possibly take longer(or more concentrated
> antibody) to stain the bone in a tissue section(mouse head, newborn
> pup, not decalcified), vs. the other tissue components(brain, skin).
> In order to get nice staining in the bone, seems like we are starting
> to see background coming up in the brain.  Wondering if this is normal
> for some antibodies.
> Thanks in advance,
> Rhonda Allen BA HT(ASCP)HTL, QIHC
> Histotechnology Specialist II
> Stowers Institute
> 1000 E. 50th Street
> Kansas City, MO 64110 
> 816-926-4305
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