Our neuropathologist wants to try a procedure he found mentioned in one
sentence, in one textbook, no reference, called "modified SDH". It stains
JUST the mitochondria in muscle fibers with "red ragged fiber" syndrome. One
of my students is taking this on as their project. 

We already do a SDH (succinic dehydrogenase) and the modified Gomori
trichrome, both of which stain normal mitochondria and those with red ragged
fibers. This modified SDH is supposed to stain only the red ragged fiber
mitochondria.

So far, we have found a few articles that mention it, and that it uses
either phenazine methosulfate or phenazine methalsufate. However, these
articles were written by PhD's or MD's, and again don't detail HOW to do the
stain, nor do they list a reference for the procedure. We've been looking
for 3 days now.

So we're turning to the wonderful people on the Histonet, knowing there is
always somone out there who does know something about every subject, and who
is willing to share their written procedure with another histotech.

Thanks.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073


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