Dear Histonetters,

I am using Neomarker's GCDFP Ab-1, Clone 23A3 at a 1:25 titer. I use a 6.0 citrate for Target Retrieval for 20 minutes in a Steamer and 20 minute cooling. I stain on the Dako Autostainer with Envision+ at 30 minutes in Primary Ab, 30 minutes in Polymer and 5 minutes in DAB. 

The staining results are just OK nothing extraordinary. 

Has anyone used this antibody and had great results?

If so, what are you doing differently than my protocol?

Do you think a 9.0 EDTA buffer for Retrieval would be better?

What about increasing my time in DAB?

Any help would be greatly appreciated.

I have emailed their techservice but thought my peers might have more to teach me.

Thanks so much,
Theresa Rohr
rohrt@nyackhospital.org

Theresa Rohr, BA HT(ASCP)
Section Head, Histology
Nyack Hospital 
160 North Midland Avenue
Nyack, New York 10960
phone - 845-348-2276
fax - 845-348-8430

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