[Histonet] Immunohistochemistry on mouse teeth
I am currently doing IHC on develping mouse teeth and I am getting only backgroung staining. The tissues are fixed O/N in 4% paraformaldehyde @4 degrees and embedded in paraffin. I have cut my sections @ 4um. I have tried HIER with Vector unmasking solution (boiling) for 2 minutes and using a kit for PCNA from Zymed. Has anyone done IHC on teeth that could give me some advice on what to try next?
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