[Histonet] Immunohistochemistry on mouse teeth
I am currently doing IHC on develping mouse teeth and I am getting only backgroung staining. The tissues are fixed O/N in 4% paraformaldehyde @4 degrees and embedded in paraffin. I have cut my sections @ 4um. I have tried HIER with Vector unmasking solution (boiling) for 2 minutes and using a kit for PCNA from Zymed. Has anyone done IHC on teeth that could give me some advice on what to try next?
Thanks!
Diane
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>