[Histonet] IHC Help!

From:"Heckford, Karen - SMMC-SF"

Dear Histonetters,  
One of my pathologists needs a antibody that will show microglandular
adenosis in human breast tissue.  I am working with FFPE tissue and the Dako
Autostainer.  I have tried Neomarkers Collagen IV Ab-3 with Dako's Envision
system.  At the time I did not have any Protease so I used Protinease K and
later used the Protease.    I did get my controls to come back positive and
my negative were negative all three times,  but the patient tissue did not
show microglandular adenosis.  We did sent this tissue out and it came back
positive for microglandular adenosis.  I cannot figure out what I am doing
wrong or perhaps I bought the wrong antibody.   

I would really appreciate any help.  This has been bothering me for a week

Karen Heckford HT (ASCP) CE
1-415-668-1000 ext. 6167

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