[Histonet] Help with IHC on mouse adipose tissue

From:"Kinsley, David"

Hi everyone,

An associate of mine is going to be working on samples of mouse adipose
tissue.  She is looking to perform IHC on paraffin sections.  Does anyone
have any experience, protocols that they would be willing to share with me?
I'm looking for appropriate tissue size, fixation conditions, processing,
and antigen retrieval conditions.   I'm sorry for the short notice but she
is planning to collect tissue samples tomorrow so I don't have much time to
research this myself.  Any suggestions are greatly appreciated.


David Kinsley
Schering-Plough Research Institute
Kenilworth, NJ 07033

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