[Histonet] HELP, Plastic sectioning

From:Sunhwa Lee

I am trying to do plastic sectioning with Fish brain and the section
is shattered a lot.  I've been changing thickness 1-5um, new knife,
and embedding materials (JB-4 and Technovit 7100), but not thing
works. My slicing conditions are as follows.  Any suggestions, please.
 In advance, thank you so much.

1. Knife : High profile stainless steel knife
2. Microtome: normal rotating Microtome (Leica 820-II)
3. Thickness: 1-5um
4. Cutting angle: 2-3degree
5. Embedding material: GMA (Technovit 7100)
6. Sample: fish brain

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