[Histonet] Attention to those doing immunofluorescent staining, looking for mounting media
Dear Histonetters doing immunofluorescence staining,
Microscopy Today 14(1):34-39, January, 2006 just published Mounting Media
and Antifade Reagents, Collins TJ
This publication is a wonderful comparison of 10 different mounting medias
for IFA work along with excellent discussion. I strongly recommend
accessing and keeping a copy of this article around. It was not an
endorsement, but certainly educational and useful.
The only thing he did not include was using diluted (in it's own solvent)
permanent mounting media (toluene or xylene based) instead of nail polish
containing isopropyl alcohol as a sealant with nail polish (cited in
literature) known to ruin GFP and cause it to not fluoresce.
My thanks to Mr. Collins!!
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-4303 (FAX)
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