[Histonet] Alkaline Phosphatase


Hi all,
Maybe you can give me some advice. I have been staining some bone tissue for alkaline phosphatase using a Bone Alk Phos antibody.  I was getting reasonable staining until recently after I started putting the tissue in a 60degree C oven to help adhere the tissue to the + slides. I was having some trouble with the sections coming off. I'm now wondering if my problem is the heat on the tissue making the AlkPhos inactive. Comments?
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