[Histonet] Alcohol Based EDTA Decalcifiers

From:"Etheridge, Sandra AL:EX"

Hello, everyone,

Is anyone aware of a commercial or homemade alcohol based EDTA
decalcifying solution?  Our fish pathologist is currently fixing his
tissues in Davidson's and would prefer to go into an alcohol decal
solution instead of an aqueous one (acid hematin
pigment forms around the red cells - not nice for photos).

Any information is, as always, greatly appreciated.

Thanks!

Sandra Etheridge

BC Ministry of Agriculture & Lands
Animal Health Center, Histology
Abbotsford, BC
Canada

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