Fwd: [Histonet] OCT embedding of NBF-fixed specimen

From:"Atoska S. Gentry"

Hello, we usually cryoprotect in 10%, 20%, & 30% Sucrose respectively in 
either the fixative which the sample was fixed or in 0.1M Phosphate Buffer. 
It really depends on the staining technique you plan to use. Allow tissue 
sample to sink in each percentage of sucrose before transferring, and after 
sample sinks in 30% it's usually safe to freeze. This technique has worked 
well for us yielding little or no ice crystal artifact. Best wishes. Atoska

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>Date: Thu, 16 Feb 2006 06:27:53 -0800 (PST)
>From: Kim Merriam 
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>Hello all,
>   I need to do some OCT embedding of tissues that are already fixed in 
> formalin.  What is the best way to do this?  Should I rinse the tissues 
> in water to get out the formalin?  How can I prevent crystal artifact on 
> these tissues?
>   Any advice would be helpful,
>   Thanks,
>   Kim
>Kim Merriam
>Cambridge, MA
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