The cases that you find nuclear staining, how long have they been fixed? My 
understanding of doing Her-2 is that the tissue has to be fixed minimum 24 
hours in 10% NBF (regardless of the size) to avoid false +/- staining. 
"Underfixation" is generally bigger problem than "overfixation" and I have 
wittnessed false nuclear staining with some antibodies.
Katri

Katri Tuomala
Hamilton, Ontario, Canada

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Sent: Monday, February 21, 2005 6:22 PM
Subject: [Histonet] her-2 nuclear staining


> Occasionally we have a her-2 with irregular nuclear staining.It seems to 
> be happening on breast needle cores.  Could it be over or under fixation? 
> If anyone has  any information I would greatly appreciate it.       D. 
> Suermann HT(ASCP)
>
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