Gudrun,

A minimum fixation time of 24 hours, in the commonly used concentration of
4% w/v formaldehyde in phosphate buffer pH 7.2-7.4, has worked well in my
hands for over 40 years of IHC, using hundreds of different antibodies.

Bryan


----- Original Message -----
From: "Gudrun Lang" 
To: "Histonetliste (Histonetliste)" 
Sent: Thursday, February 17, 2005 3:12 PM
Subject: [Histonet] formaldehyd concentration and IHC


> In addition to the fixation-time-discussion a question about the
> concentration. What formaldehyd concentration do you prefer to get the
> optimal IHC- result?
>
> 4% or higher?; or does it really matter?
>
>
>
> Gudrun Lang
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet