Re: [Histonet] Vibratome info

From:Roger Moretz

Could you describe more clearly the problems you are
having?  I have successfully used a vibratome for
years (and sporadically at that), always getting 50um
sections of the samples I am sectioning.  Have done
everything from cns to liver and kidney and nearly
everything else in between.  Successfully mounting
your samples is the first goal.  Second, I have always
preferred a #22 scalpel blade to a razor blade--using
only the straight portion of the blade of course. 
Third, maintaining a cool temperature in the bath
(with ice or the chiller if the vibratome is so
equipped) is essential.  Finally, don't try to do
mammoth samples--altho I have done up to 25x25mm
samples without a problem.  If you have done all of
these, please provide more details and I'll see if I
can help.

Roger Moretz, Ph.D
Dept of Toxicology
Boehringer Ingelheim Pharmaceuticals

--- Cheryl Crowder 

> Hi all - One research lab is doing confocal
> microscopy.  They were given a 
> virtatome and are trying (without success) to get 50
> um sections.  If any of 
> you know the intricasies of the virbatome and are
> willing to correspond or 
> talk to the tech that is trying to work with the
> virbatome, would you 
> contact me and I will pass the information on.   I
> know that she has the 
> e-mail address and phone number for Virbratome, but
> would also like to have 
> contact with working techs.   Thank you in advance, 
> Cheryl   
> Cheryl Crowder, BA, HTL(ASCP)
> Chief Technologist
> Anatomic Pathology
> Department of Pathobiological Sciences
> School of Veterinary Medicine
> Louisiana State University
> Skip Bertman Drive
> Baton Rouge, LA  70803
> 225-578-9734
> FAX:  225-578-9720
> _______________________________________________
> Histonet mailing list

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