Re: [Histonet] Sudan Black B

From:Bryan Llewellyn

They are also called "sintered glass filters".

They look like a large filter funnel with a rough glass plate permanently 
bonded just above the constriction.  The rough glass plate is made of glass 
fibres or particles partially melted together to give the desired pore size. 
We used to use them for filtering our diluent for white cell counts on early 
model Coulter cell counters (in 1962 when I was a student in hematology). 
They were used because they could be put under vacuum without distorting and 
large volumes of the fluid could be filtered fast and efficiently.

Bryan Llewellyn

----- Original Message ----- 
From: "Danielle Crippen" 
Sent: Thursday, February 17, 2005 11:03 AM
Subject: [Histonet] Sudan Black B

Hi all,

I'm hoping to find some help with the protocol for this lipofuscin stain. 
It instructs to "filter through filter paper and then filter again through a 
frittered glass filter of medium porosity with suction".

Can anyone define "frittered glass filter" for me??  I have my guesses, but 
I prefer to consult the experts first.

Many thanks in advance!!

Danielle Crippen

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