Re: [Histonet] Block

From:Shawnster73@aol.com

 
My antibody is a rabbit polyclonal, but the tissue is mouse xenograft  tissue 
of human prostate cancer.
 
 
In a message dated 2/23/2005 9:06:59 AM Eastern Standard Time,  
anh2006@med.cornell.edu writes:

Is your  antibody a mouse monoclonal? More info is needed before I can  help.


>I am currently trying to stain mouse xenograft tissue  using a p53 
>antibody.  I have worked up this antibody on human  breast cancer 
>using the Ventana AB block and amplifier and have gotten  beautiful 
>results.  I am now looking for a way to block and  amplify the tissue 
>using reagents that do not contain mouse.  Any  suggestions would be 
>greatly appreciated.
>
>Michelle  HT(ASCP)
>
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