Teri - We have a Dako Autostainer in our lab which is strictly research no service and find it very flexible and user friendly.  We still do all of our overnight incubations at 4C with the exception of Dystrophin antibodies which we do at room temperature.  We do all of our substrate incubations on the instrument - using standardized system protocols.  The Dako is nice due to the fact it allows you to dispense at three different areas on the slide using a minimum amount of 100 microliters in each position on the slide.  Hope this helps out.

"Johnson, Teri"  wrote:Hi all (and apologies to those on both lists),

For those of you doing research histology and immunohistochemistry,
which automated immunostainer do you use? Do you do any overnight
incubations with it? For those of you who have automated stainers and
formerly did overnight (4C) incubations, what modifications did you make
to your staining protocol to achieve the same result? Also, do you
always use the substrate on instrument, or do you do that via
microscope?

Some immunostainers require minimum amounts of primary antibody (~500
microliters), even if staining only one slide. Some times we don't have
that much antibody to use. Are there any tips to get around this with
the exception of manually pipetting as if doing a titration run?

Thanks for your input!


Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
tjj@stowers-institute.org



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Amy S.Porter, HT(ASCP), QIHC
Michigan State University - Department of Physiology
Division of Human Pathology 
Investigative Histopathology Laboratory
2201 Biomedical Physical Sciences  Room 2133
East Lansing, MI  48824-3320
Ph:  517-355-6475 ext 1480/1481
Fax:  517-432-1368
portera@msu.edu



		
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