RE: [Histonet] antigen retrieval question
If I remember correctly, mine was an anti-collagen I or II that did not work
after sitting in buffer overnight. I also know that one of my part-timers
tried this at the hospital he works at and they also had disastrous results
on day #2.
______________________
Jim Staruk
Mass Histology Service
www.masshistology.com
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Friday, February 18, 2005 7:42 AM
To: Jim Staruk; 'Patsy Ruegg'; ihcrg@neo.agsci.colostate.edu
Cc: histonet@pathology.swmed.edu
Subject: RE: [Histonet] antigen retrieval question
Patsy,
I have done this many times before, and have never had any issues with
re-masking. Why would the sites be re-masked if they are not subjected to
formalin again? If I am staining a big batch of slides (anything over 100
slides at a time), I do the depar and retrieval on the first day and all of
the antibody stuff on the second day. Maybe it just depends on the
protein/antibody?
This has peaked my interest. I am curious to see if anyone else has
encountered this problem.
Kim Merriam
Novartis
Cambridge, MA
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