We had some success using 2N HCl at 37deg C for 30 minutes. This was enough
to uncover the epitope and sections stayed on slides.
If you are cutting more sections try using pre-coated (poly-L?) or charged
slides (Superfrost Plus) as they help sections stick during IHC 

Andrew

Andrew Prior
Histologist
Smith & Nephew Research Centre
York Science Park
Heslington
York




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