We've always done our HIER before the EIER but mainly because that's how our instruments proceed.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
bliven.laura@marshfieldclinic.org
Sent: Wednesday, February 16, 2005 1:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HIER and EIER



When used in combination, is it better to use HIER (Heat Induced Epitope Retrieval) first or EIER (Enzyme Induced Epitope Retrieval) first.  Or does it depend upon the antigen of interest?

Any references?

Thanks,
Laura Bliven


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