RE: [Histonet] HIER and EIER

From:"Sebree Linda A."

We've always done our HIER before the EIER but mainly because that's how our instruments proceed.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
600 Highland Ave.
Madison, WI 53792
FAX:  (608)262-7174

-----Original Message-----
[]On Behalf Of
Sent: Wednesday, February 16, 2005 1:01 PM
Subject: [Histonet] HIER and EIER

When used in combination, is it better to use HIER (Heat Induced Epitope Retrieval) first or EIER (Enzyme Induced Epitope Retrieval) first.  Or does it depend upon the antigen of interest?

Any references?

Laura Bliven

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