RE: [Histonet] Formalin Fixation times for IHC samples

From:"Connolly, Brett M"

Take a look at the J. Histotechnology 24:3, Sept 2001....special issue on
Fixation. It includes a couple of articles dealing with fixation and IHC.


Brett M. Connolly, Ph.D.
Merck & Co., Inc.
MRL, Imaging Research
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-652-2075

-----Original Message-----
[] On Behalf Of
Sent: Thursday, February 24, 2005 9:06 AM
Subject: RE: [Histonet] Formalin Fixation times for IHC samples

Great timing on this topic

I have been looking for a review article that actually puts in print the
data collected on over fixation or under fixation in samples that will be
used for IHC. To include the eventual breakdown of formalin and need for
10-20 times volume for proper fixation. Is there such a paper with all of
information that histologist reverberate and others ignore?

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