RE: [Histonet] Formalin Fixation times for IHC samples

From:Browning Deb

24 hours works best for us, we have standardized the retrieval protocols
based on this period of fixation.

-----Original Message-----
From: Anne C Lewin [mailto:anne.lewin@bms.com]
Sent: Tuesday, February 15, 2005 10:28 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formalin Fixation times for IHC samples


Could I get a general concensus from Histoland about the amount of time 
needed to fix a sample for IHC without over-fixing?  My training/journal 
searches  have led  me to believe that the longer a sample is in 
formalin, the stronger the protiens are cross-linked, and the more 
difficult it is to break those bonds with antigen retreival and get your 
antibody to the target.  I generally tell the scientists in my 
department to fix overnight (most samples vary from about the size of a 
pea to a lima bean), and then to transfer to 70% Ethanol for me to 
process for paraffin.  The total time in fixative tends to be around 24 
hours.  Morphology has always been great, and my IHC's work well.  This 
question is mainly for my information, since I have used the same tissue 
prep protocol for a few years now, I want to make sure I am keeping up 
with current opinions.
Thanks!
-Anne

This information is directed in confidence solely to the person named above
and may not otherwise be distributed, copied or disclosed.  Therefore, this
information should be considered strictly confidential.  If you have
received this email in error, please notify the sender immediately via a
return email for further direction. Thank you for your assistance.



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>