David,
Don't have protocols to hand - but is roughly 8 hours for each step of
dehydration.
Need to know what your final use for the bone is? Are you embedding and
sectioning it or just doing gross pathology?

As for the saw, we have just acquired an Exakt cutting system, and the band
saw is excellent. Cuts thin (200 micron easy) MMA sections with very little
damage to tissue, haven't tried fresh tissue yet. website is www.exakt.de
The guys are very helpful. They are having a practical workshop at the start
of April which you may be interested in so you can see their stuff ( I'm not
employed by them, just happy with the new toys/ equipment in the lab).

Andrew

Andrew Prior
Histologist
Smith & Nephew Research Centre
York Science Park
Heslington
York

Date: Wed, 16 Feb 2005 08:10:20 -0000
From: "Muskett David" 
Subject: [Histonet] Bone saw and bone protocols[Scanned]
To: 
Message-ID:
	<6AB79F0BA7C4914BA8DD8F5E0C21B9752E2928@AHEXMAIL01.xalderhey.com>
Content-Type: text/plain;	charset="US-ASCII"

Dear All
 
I am currently reviewing our protocols for the processing of bone. Would
anybody be willing to share protocols and processing schedules?
 
I am also interested in buying a bone saw, preferably one which allows
thin sections to be cut easily. Can anybody recommend me a firm or a
saw?
 
Thanks
 
David

David Muskett
Chief Biomedical Scientist, Histology
Royal Liverpool Children's NHS Trust
Eaton Road
Liverpool
L12 2AP
Internal telephone x3615
Telephone (0151) 293 3656
Fax (0151) 293 3617
http://nhsald02/histopathology  

 





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