We are looking into submitting for biopsy, all of our placentas. We are a
large merged hospital with many outreach stations. I would like to know if
anyone out there is currently dealing with grossing, sectioning, and storing
of these specimens. I would like to know if you are using fixed and/or fresh
tissue and if you are ultimately providing PCR and genetic testing.
Please contact me either by email or phone. Any information will be greatly
appreciated.

Annette Featherstone HT/MLT
Supervisor, Anatomic Pathology
Kaleida Health
Buffalo General Hospital
100 High St
Buffalo NY 14203
716-859-2625


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Prior,
Andrew
Sent: Wednesday, February 16, 2005 10:19
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Re: Bone saw and bone protocols


David,
Don't have protocols to hand - but is roughly 8 hours for each step of
dehydration.
Need to know what your final use for the bone is? Are you embedding and
sectioning it or just doing gross pathology?

As for the saw, we have just acquired an Exakt cutting system, and the band
saw is excellent. Cuts thin (200 micron easy) MMA sections with very little
damage to tissue, haven't tried fresh tissue yet. website is www.exakt.de
The guys are very helpful. They are having a practical workshop at the start
of April which you may be interested in so you can see their stuff ( I'm not
employed by them, just happy with the new toys/ equipment in the lab).

Andrew

Andrew Prior
Histologist
Smith & Nephew Research Centre
York Science Park
Heslington
York

Date: Wed, 16 Feb 2005 08:10:20 -0000
From: "Muskett David" 
Subject: [Histonet] Bone saw and bone protocols[Scanned]
To: 
Message-ID:
	<6AB79F0BA7C4914BA8DD8F5E0C21B9752E2928@AHEXMAIL01.xalderhey.com>
Content-Type: text/plain;	charset="US-ASCII"

Dear All
 
I am currently reviewing our protocols for the processing of bone. Would
anybody be willing to share protocols and processing schedules?
 
I am also interested in buying a bone saw, preferably one which allows
thin sections to be cut easily. Can anybody recommend me a firm or a
saw?
 
Thanks
 
David

David Muskett
Chief Biomedical Scientist, Histology
Royal Liverpool Children's NHS Trust
Eaton Road
Liverpool
L12 2AP
Internal telephone x3615
Telephone (0151) 293 3656
Fax (0151) 293 3617
http://nhsald02/histopathology  

 





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