[Histonet] Negative Rabbit Control

From:"Coskran, Timothy M"

For those using negative Rabbit IgG, how do you determine the dilution of
your negative.  Are you matching the IgG concentration of your negative
rabbit IgG to that of the primary antibody at its working dilution?  What do
you do when a vendor does not know the IgG concentration of a rabbit
polyclonal primary antibody?

We try to match the isotype and Ig concentration for negative controls when
using monoclonals, but this seems to be a difficult task for rabbit
polyclonal antibodies for which you don't have a starting Ig concentration.

Tim Coskran

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