[Histonet] Dry paraformaldehyde vapor
In order to avoid tritium contamination of screens, I have been fixing
my slides with paraformaldehyde vapor. PF powder is placed in the bottom
of a dessicator with slides. Air is removed from the dessicator and the
air-tight chamber exposes the tissue to paraformaldehyde overnight. Has
anyone used this method? I am having a hard time understanding why the
powder alone is sufficient for fixation. Liquid fixative is not an
option because the receptor/lingand complex is washed off. The protocol
is vaguely outlined in Biotechiques 26:432-434; Liberatore, et. al. 1999.
Thanks for your input,
Kelly
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