[Histonet] Can TUNEL be used for in situ Double Strand Break detection assay?

From:"P. Krawczyk"

I was wondering whether I could use the TUNEL / ISOL assay to label DNA
double strand breaks specificaly. I know, that the terminal transferase
(TUNEL) also labels single strand breaks and thus the staining would be
probably non-specific. But I also heard that the T3 DNA Ligase (ISOL) labels
duplex DNA breaks exclusively. Does anybody have experience with this type
of labeling? Can it be used to visualize single DNA breaks under
fluorescence microscope?
Thank you,
P. Krawczyk
P. M. Krawczyk
Dept. of Cell Biology and Histology
Academic Medical Centre
University of Amsterdam
Meibergdreef 15, room M3-107
1105 AZ, Amsterdam
The Netherlands
Email:  P.Krawczyk@amc.uva.nl
Tel:     +31 20 5668746
Fax:    +31 20 6974156
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