[Histonet] Automated immunostainer for research applications

From:"Johnson, Teri"

Hi all (and apologies to those on both lists),
For those of you doing research histology and immunohistochemistry,
which automated immunostainer do you use?  Do you do any overnight
incubations with it?  For those of you who have automated stainers and
formerly did overnight (4C) incubations, what modifications did you make
to your staining protocol to achieve the same result?  Also, do you
always use the substrate on instrument, or do you do that via
Some immunostainers require minimum amounts of primary antibody (~500
microliters), even if staining only one slide.  Some times we don't have
that much antibody to use.  Are there any tips to get around this with
the exception of manually pipetting as if doing a titration run?
Thanks for your input!

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110

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