Re: [Histonet] fluorescent counterstain
There are many. My favourite is neutral red
(CI 40040; Basic red 5). Stain sections for
5 min with a 0.002% aqueous solution, then
dehydrate, clear and cover. Use a non-fluorescent
mountant such as DPX. Orange-red fluorescence
with a wide range of workable excitation
wavelength (325-500 nm). For more information
and a picture, see Neuroscience 99: 755-764 (1994).
John Kiernan
London, Canada
---------------------------------------------------
Susan Travers wrote:
>
> Can anyone recommend a fluorescent counterstain for cell bodies in
> neural tissue (similar to cresyl violet). The tissue is formalin
> fixed.
>
> Thanks
> Susan Travers
> --
> Susan Travers, Ph.D.
> Professor, Oral Biology
>
> The Ohio State University
> College of Dentistry
> 305 W. 12th Avenue
> Columbus, Ohio 43210-1267
>
> (614)292-6366(V)
> (614)247-6945(F)
>
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