Re: [Histonet] cresyl violet staining on unfixed frozen brain sections
When I did this method (admittedly on paraffin sections) years ago, I used
only a few drops of acetic acid in a staining dish (200-300 mL) of 95%
ethanol. However, integral to the method was dehydrating and clearing with
xylene before differentiation. The method I used specified to stain, rinse
with water, dehydrate in ethanols and clear with xylene, then leave
overnight in xylene before differentiating. This took too long, so I used
to leave the slides about 30 minutes. I found that it did help to remove
dye from the background faster than from the cells and improved contrast.
Since the sections have already been dehydrated, the post-differentiation
dehydration can be gentler.
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